Experimental evaluation of the influence of gold nanoparticles and cerium dioxide on normal and malignant cells and tissues

Volume 4, Issue 3, June 2020     |     PP. 44-54      |     PDF (160 K)    |     Pub. Date: August 12, 2020
DOI:    215 Downloads     5919 Views  

Author(s)

Reznikov A.G., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Faliush O.A., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Nosenko N.D., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Sachynska O.V., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Polyakova L.I., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Limareva A.A., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Perchyk I.G., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine

Abstract
Presented are the results of experimental studies of the effect of gold nanoparticles (AuNP) on reproductive organs of male rats, human cells and tissues of androgen-dependent prostate cancer (PCa), as well as effect of cerium dioxide nanoparticles (CeO2NP) on the testes and fertility in aging male rats. Presence of polydisperse colloidal solution of AuNP (10-50 nm) in the LNCaP culture medium at a final concentration of 10 μg/ml inhibited cell growth, while the monodisperse solution of AuNP (20 nm) had no effect. The polydisperse colloidal AuNP solution arrested the growth of human PCa xenografts in mice, when administered parenterally at a dose range of 0.64-6.4 μg/kg b.w. The selectivity of the the nanopreparations effect on the prostate malignant epithelium is confirmed by its destruction and a decrease in the epithelial-stromal ratio on histological preparations of the xenografts. There was no significant damaging effect of poly- and monodisperse AuNP solutions on the testes and accessory sexual glands of rats when administered for up to two weeks. The stimulating effect of administered orally a low dose of CeO2NP (1 mg/kg b.w.) on testosterone secretion and spermatogenesis, proliferative and secretory processes in the prostate of aging male rats was found. However, fertility of animals reduced in comparison with the control group due to immaturity of a part of the spermatozoa. The mechanisms of the stimulating or damaging effects of metal nanoparticles and their salts on normal and malignant tissues require further research in order to evaluate their therapeutic potential and toxicity.

Keywords
gold nanoparticles, cerium dioxide nanoparticles, reproductive system, aging, prostate cancer

Cite this paper
Reznikov A.G., Faliush O.A., Nosenko N.D., Sachynska O.V., Polyakova L.I., Limareva A.A., Perchyk I.G., Experimental evaluation of the influence of gold nanoparticles and cerium dioxide on normal and malignant cells and tissues , SCIREA Journal of Medicine. Volume 4, Issue 3, June 2020 | PP. 44-54.

References

[ 1 ] Boulaiz H, Alvarez PJ, Ramirez A. Nanomedicine: Application Areas and Development Prospects. Int J Mol Sci. 2011;12(5):3303-3321.
[ 2 ] Paton BYe, Moskalenko VF, Checkman IS. Nanoscience and Nanotechnology: Technical, Medical and Social Aspects. Visnyk of the National Academy of Sciences of Ukraine. 2009;6:18-29.
[ 3 ] Maslyakova GN, Pakhomiy SS, Buchar AB. Morphological changes in the organs of laboratory animals with prolonged oral administration of gold nanoparticles. Saratov Journal of Medical Scientific Research. 2013;9(2):208-213.
[ 4 ] Pocheptsov AYa, Magnificent YuI, Filatov BN. Effect of gold nanoparticles on the proliferative activity of rat germ cells. Vestnik Volzhskogo universiteta im. V.N. Tatishcheva. 2012;42(2):47-50.
[ 5 ] Chekman IS. Nanopharmacology. K.:Zadruha. 2011;424.
[ 6 ] Dykman LA, Khlebtsov NG. Gold Nanoparticles in Biology and Medicine: Recent Advances and Prospects. Acta Naturae. 2011;3(2):34-55.
[ 7 ] Giasuddin AM, Jhuma KA, Mujibul AM. Use of Gold Nanoparticles in Diagnostics, Surgery and Medicine: A Review. Bangladesh J Med Biochem. 2012;5(2):56-60.
[ 8 ] Arnida, Malugin A, Ghandehari. Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres. J Appl Toxicol. 2010;30(3):212-217.
[ 9 ] Zholobak NM, Olevinskya ZM, Spivak NYa. Antiviral effect of cerium dioxide nanoparticles stabilized by low-molecular polyacrylic acid. Mikrobiolohichnyi zhurnal. 2010;72(3): 42-47.
[ 10 ] Spivak NYa, Nosenko ND, Zholobak NM. The nanocrystalline cerium dioxide raises the functional activity on genesial system of ageing males of rats. Nanosystems: Physics, Chemistry, Mathematics. 2013;4(1):72-77.
[ 11 ] Shcherbakov AB, Ivanov VK, Zholobak NM. Nanocrystaline ceria based materials – perspectives for biomedical application. Biophysics. 2011;56(6):995-1015.
[ 12 ] Pat 87744 U UA, МПК А61К 31/79, А61К 33/00. A composition containing gold nanoparticles, and a method for its preparation / OV Usatenko, OB Shcherbakov, NF Kushchevskaya, GV Push; Applicant and Patent Owner OV Usatenko - No. 2007200612; Stated. November 14, 2007; Has published Aug 10, 2009, Bul. No. 15.
[ 13 ] Reznikov AG, Salivonyk OA, Sotkis AG. Assessment of gold nanoparticle effect on prostate cancer LNCaP cells. Exp Oncol. 2015;37(2):100-104.
[ 14 ] Li Y, Chen X, Gu N. Computational investigation of interaction between nanoparticles and membranes: hydrophobic/hydrophilic effect. J Phys Chem B. 2008;112(51):16647-16653.
[ 15 ] Roiter Y, Ornatska M, Rammohan AR. Interaction of lipid membrane with nanostructured surfaces. Langmuir. 2009;25(11):6287-6299.
[ 16 ] Reznichenko LS. Biochemical effects of the influence of gold nanoparticles on prokaryotic and eukaryotic cells. PhD Dissert. Thes. 2010;148.
[ 17 ] Dibkova CM, Reznichenko LS, Gruzina TG. Evaluation of in vivo DNA-damaging action of gold nanoparticles of different sizes. Biotechnologia Acta. 2010;3(3):66-71.
[ 18 ] Stavrovska AA. Cellular Mechanisms of Multiple Drug Resistance of Tumor Cells. Stavrovsk. Biochemistry. 2000;65(1):112-126.
[ 19 ] Falyush OA, Polyakova LI, Sachynska ОV. The changes of epithelial-stromal ratio in transplants of human prostate cancer under application of gold nanoparticles. Pharmacology and drug toxicology. 2016;50(4-5):86-89.
[ 20 ] Reznikov AG, Salivonyk OA, Sachinskaya OV. Investigation of the effects of various doses of gold nanoparticles on prostate cancer xenografts. Urology. 2014;18(4):17-22.
[ 21 ] Zakhidov ST. Effect of gold nanoparticles on mouse spermatogenesis. Biology bulletin. 2012;39:229-236.
[ 22 ] Zhou W, Gao X, Liu D. Gold Nanoparticles for In Vitro Diagnostics. Chem Rev. 2015;115(16):10575-10636.
[ 23 ] Petrovskaya YeG. Application of nanotechnology in medicine and pharmacology. Ethics of nanotechnologies and nanosecurity. Proceedings of the international seminar. 2014;3;32-34.
[ 24 ] Trahtenberg ІМ, Ulberg ZR, Chekman IS. Substantiation of expediency of creation and introduction of normative-methodical basis of safety evaluation of medical nanoparticles in Ukraine. Scientific Journal of the Ministry of Health of Ukraine. 2014;2:20-26.
[ 25 ] Falyush OA, Sachynska ОV, Polyakova LI. Description of the reproductive organs of male rats after application of a monodispersed solution of gold nanoparticles. Endocrinology. 2016;21(3):213-219.
[ 26 ] Saleh HM, Soliman OA, Elshazlyn MO. Acute hematologic, hepatologic, and nephrologic changes after intraperitoneal injections of 18 nm gold nanoparticles in hamsters. Int J Nanomed. 2016;11:2505-2513.
[ 27 ] Salivonyk OA, Sachinskaya OV, Polyakova LI. Effect of gold nanoparticles on reproductive organs of male rats. Clinical and experimental pathology. 2015;14(2):176-179.
[ 28 ] Filatov BN, Bocharova LI, Klauschek VV. Production and application of nanomaterials (toxicological and hygienic problems). www.MEDLINE.RU. 2015;16:259-266.
[ 29 ] Dementieva OV, Evdokimov YuM, Zahidov ST. The influence of gold nanoparticles on the spermatogenesis of mice. Biology Bulletin. 2012;3:279-287.
[ 30 ] Vandooren J, Berghmans N, Dillen C. Intradermal air pouch leukocytosis as an in vivo test for nanoparticles. Int J Nanomed. 2013;8(1):4745-4756.
[ 31 ] Villiers C, Freitas H, Couderc R. Analysis of the toxicity of gold nanoparticles on the immune system: effect on dendritic cell functions. Nanopart Res. 2010;12(1):55-60.
[ 32 ] Nosenko ND, Zholobak NM, Polyakova LI. Morphofunctional state of the reproductive system of rats in the aging period under the conditions of use of nanoscale. Fiziolohichnyi zhurnal. 2014;60(1):11-17.