ISSN: 2706-8870
Volume 9, Number 3 (2024)
Year Launched: 2016
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The role of adhesion molecule expression in the development of morphological changes in renal tissue in patients with type 2 diabetes

Volume 9, Issue 3, June 2024     |     PP. 91-115      |     PDF (368 K)    |     Pub. Date: August 31, 2024
DOI: 10.54647/cm321287    32 Downloads     2561 Views  

Author(s)

T.S. Ryabova, S.M. Kirov Military Medical Academy, Department of Nephrology and Efferent Therapy, 194044, Saint Petersburg, st. Academician Lebedeva, 6, Russia
I.A. Rakityanskaya, Department of Allergology, Immunology and Clinical Transfusiology, Municipal Outpatient Hospital no. 112, 195427, Saint Petersburg, st. Academician Baykova, 25/1, Russia

Abstract
Research objective. The work is devoted to studying the role of the expression of intercellular adhesion molecules-1 (ICAM-1, CD54), P-selectin (CD62P) and E-selectin (CD62E) in renal tissue in the development and progression of morphological changes in diabetic nephropathy in patients with type 2 diabetes mellitus.
Material and methods. The examination was carried out in 50 patients with type 2 diabetes mellitus (T2DM) (average age 66.58±3.27 years). The duration of diabetes was 14.744±0.1.062 years. All patients underwent intravital puncture biopsy of the kidney. To confirm the morphological diagnosis, light and immunofluorescence microscopy of kidney tissue biopsies were performed. Morphological changes in tissue were assessed in accordance with the latest international classification of diabetic nephropathy, developed in 2010. According to light microscopy, class IIa (mild mesangial expansion) was identified in 12 patients, class IIb (severe mesangial expansion) was identified in 14 patients, in 19 patients – class III (nodular Kimmelstiel-Wilson lesions) and in 5 patients – class IV (advanced diabetic glomerulosclerosis).
The expression of ICAM-1, P- and E-selectin was determined using monoclonal antibodies, FITC anti-human CD54 Antibody, FITC anti-human CD62P Antibody, FITC anti-human CD62E Antibody (USA). The intensity of expression in points (0–4), the nature and location of the expression of adhesion molecules in the glomerular endothelium and in peritubular capillaries were assessed.
Results. To identify the prognostic significance of the expression of CD54, CD62R and CD62E in the progression of morphological changes in tissue during the development of DN, correlation analysis and linear regression analysis were performed. The findings demonstrated the role of adhesion molecule expression in the development of mesangial matrix expansion, basement membrane thickening, arteriolar hyplinosis, and tubulointerstitial lesions. The development of morphological changes in tissue is also confirmed by the regression model between the expression of adhesion molecules in tissue with the progression of the stage of DN.
Conclusion. We conducted a statistical analysis to explore how the expression of adhesion molecules affects the changes in kidney tissue. This analysis confirmed the previously suggested idea that interstitial damage plays a crucial role in the progression of DKD. One of the main mechanisms contributing to the development of interstitial fibrosis is the expression of certain adhesion molecules, such as ICAM-1, CD62P, and CD62E

Keywords
Type 2 Diabetes Mellitus, Diabetic Kidney Disease, Renal Biopsy, ICAM-1, P-selectin, E-selectin

Cite this paper
T.S. Ryabova, I.A. Rakityanskaya, The role of adhesion molecule expression in the development of morphological changes in renal tissue in patients with type 2 diabetes , SCIREA Journal of Clinical Medicine. Volume 9, Issue 3, June 2024 | PP. 91-115. 10.54647/cm321287

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